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biotinylated goat anti mouse c3d  (R&D Systems)


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    R&D Systems biotinylated goat anti mouse c3d
    Biotinylated Goat Anti Mouse C3d, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+c3d/Mouse+Complement+Component+C3d+Biotinylated+Antibody/pm41810513-65-15-18
    Average 93 stars, based on 30 article reviews
    biotinylated goat anti mouse c3d - by Bioz Stars, 2026-10
    93/100 stars

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    Marker:

    Article Title: Mitochondrial Intercellular Transfer via Platelets After Physical Training Exerts Neuro‐Glial Protection Against Cerebral Ischemia
    Article Snippet: .. After additional PBS washes, cells were blocked with 2% Blockace in PBS for 1 h. The following primary antibodies were used: goat polyclonal anti‐C3d (1:50; R&D Systems; AF2655), rabbit polyclonal anti‐S100A10 (1:100; Proteintech; 11250‐1‐AP), rabbit polyclonal anti‐PDGFRα (1:100; Santa Cruz Biotechnology; sc‐338), and mouse monoclonal anti‐myelin basic protein (MBP; OLG marker, 1:50; Millipore; MAB382). .. Following incubation with primary antibodies, cells were washed with PBS containing 0.2% Tween‐20 and incubated with appropriate secondary antibodies (1:200; Jackson ImmunoResearch Laboratories) for 1 h at room temperature.

    Article Title: Mitochondrial Intercellular Transfer via Platelets After Physical Training Exerts Neuro‐Glial Protection Against Cerebral Ischemia
    Article Snippet: Sections were blocked with 2% Blockace (Dainippon Sumitomo Pharma, Osaka, Japan; UK‐B80) for 1 h at room temperature, followed by overnight incubation at 4°C with primary antibodies. .. The following primary antibodies were used: mouse monoclonal anti‐GFAP (astrocyte marker; 1:200, Santa Cruz Biotechnology; sc‐33673), rabbit polyclonal anti‐GST‐pi (OLG marker; 1:200, MBL; code 312), mouse monoclonal anti‐Olig1 (OLG marker; 1:100, Millipore; MAB5540), mouse monoclonal anti‐CNPase (OLG marker; 1:100, Abcam; ab6319), rabbit polyclonal anti‐PDGFRα (OPC marker; 1:100, Santa Cruz Biotechnology; sc‐338), mouse monoclonal anti‐PDGFRα (1:100, Santa Cruz Biotechnology; sc‐398206), mouse monoclonal anti‐CD140a (OPC marker; 1:100, BD Biosciences; 558774), goat polyclonal anti‐C3d (inflammatory astrocyte marker; 1:50, R&D Systems; AF2655), rabbit polyclonal anti‐S100A10 (protective astrocyte marker; 1:100, Proteintech; 11250‐1‐AP), mouse monoclonal anti‐NeuN (neuronal marker; 1:100, Millipore; MAB377), mouse monoclonal anti‐SMI31 (axonal marker; 1:100, BioLegend; 801601), rabbit polyclonal anti‐Iba1 (microglial marker; 1:200, Wako; 019–19741), rat monoclonal anti‐CD31 (endothelial marker; 1:100, BD Pharmingen; 550274), and mouse monoclonal anti‐TOM20 (mitochondrial marker; 1:100, BD Transduction Laboratories; 612278). .. After washing with PBS, sections were incubated with appropriate secondary antibodies (1:250, Jackson ImmunoResearch Laboratories) for 1 h at room temperature, followed by mounting with VECTASHIELD containing DAPI (Vector Laboratories; H‐1200).

    Article Title: Mitochondrial Intercellular Transfer via Platelets After Physical Training Exerts Neuro‐Glial Protection Against Cerebral Ischemia
    Article Snippet: .. The following primary antibodies were used: mouse monoclonal anti‐GFAP (1:2000; Santa Cruz Biotechnology; sc‐33673), goat polyclonal anti‐C3d (1:1000; R&D Systems; AF2655), rabbit polyclonal anti‐S100A10 (1:2000; Proteintech; 11250‐1‐AP), mouse monoclonal anti‐MBP (1:2000; Thermo Scientific; MAB382), rabbit polyclonal anti‐PDGFRα (1:2000; Santa Cruz Biotechnology; sc‐398206), mouse monoclonal anti‐TOM20 (1:1000; BD Transduction Laboratories; 612278), rabbit polyclonal anti‐SOD2 (1:5000; proteintech; 24127‐1‐AP), mouse monoclonal anti‐NeuN (neuronal marker, 1:2000; Millipore; MAB377), mouse monoclonal anti‐SMI31 (axonal marker, 1:2000; BioLegend; 801601), rabbit polyclonal anti‐Iba‐1 (1:2000; Wako; 019–19741), and mouse monoclonal anti‐β‐actin (1:10000; Abcam; ab8226). .. Following overnight incubation at 4°C with primary antibodies, membranes were washed and incubated with HRP‐conjugated secondary antibodies (1:5000; Santa Cruz Biotechnology).

    Incubation:

    Article Title: Intermittent theta-burst stimulation promotes neurovascular unit remodeling after ischemic stroke in a mouse model
    Article Snippet: Next, the 2- to 3-mm outer contours of the brain sections were incubated with a general-purpose antibody diluent containing 0.3% TritonX-100 (Sigma-Aldrich) for 20 minutes at room temperature followed by 1 hour of incubation at room temperature with bovine serum albumin. .. Next, the sections were incubated overnight at 4°C with the following primary antibodies: anti-phospho-AKT [Ser473] (rabbit, 1:300, Affinity, Cat# AF0016, RRID: AB_2810275), anti-phospho-GSK3β[Ser9] (rabbit, 1:300, Affinity, Cat# AF2016, RRID: AB_2834439), anti-NeuN (mouse, 1:300, Abcam, Cambridge, UK, Cat# ab104224, RRID: AB_10711040), antiNeuN (rabbit, 1:300, Proteintech, Cat# 26975-1-AP, RRID: AB_2880708), antiBDNF (rabbit, 1:300, Affinity, Cat# DF6387, RRID: AB_2838350), anti-BCL-2 (rabbit, 1:200, Proteintech, Cat# 12789-1-AP, RRID: AB_2227948), anti-C3d (goat, 1:200, R&D Systems, Cat# AF2655, RRID: AB_2066622), anti-S100A10 (rabbit, 1:200, Proteintech, Cat# 11250-1-AP, RRID: AB_2269906), antiGFAP (rabbit, 1:300, Proteintech, Cat# 16825-1-AP, RRID: AB_2109646), antiGFAP (mouse,1:300, Cell Signaling Technology, Cat# 3670, RRID:AB_561049), anti-Iba1 (rabbit, 1:300, FUJIFILM, Wako, Japan, Cat# 019-19741, RRID: AB_839504), anti-Iba1 (mouse,1:300, FUJIFILM, Cat# 011-27991, RRID: AB_2935833), and anti-CD31 (goat, 1:200, R&D Systems, Cat# AF3628, RRID: AB_2161028). .. After primary antibody incubation, the sections were washed with 1× PBS three times for 5 minutes each and incubated with Alexa Fluor 488 (goat, 1:1000, Abcam, Cat# ab150077, RRID: AB_2630356), Alexa Fluor 594 (goat, 1:1000, Abcam, Cat# ab150116, RRID: AB_2650601), and Alexa Fluor 647 (donkey, 1:1000, Abcam, Cat# ab150131, RRID: AB_2732857) secondary antibody at room temperature for 1.5 hours.

    Article Title: The GLP1R Agonist Semaglutide Inhibits Reactive Astrocytes and Enhances the Efficacy of Neural Stem Cell Transplantation Therapy in Parkinson's Disease Mice
    Article Snippet: Electrophoresis was conducted by a 10% SDS‐polyacrylamide gel, and transferred protein samples were transferred to PVDF membranes (Millipore, Cat# IPVH00010). .. The following primary antibodies were used for incubation overnight at 4 °C: rabbit anti‐beta‐actin (Proteintech, China Cat# 81115‐1‐RR), rabbit anti‐tyrosine hydroxylase (TH) (Proteintech, China, Cat#25859‐1‐AP), rabbit anti‐GFAP (Proteintech, China, Cat#16825‐1‐AP), and goat anti‐C3d (R&D Systems, CatAF2655). .. All membranes were incubated with HRP‐conjugated Affinipure Rabbit Anti‐Goat IgG (H+L) (Proteintech, China, Cat#RGAM001) or HRP‐conjugated Affinipure Goat Anti‐Rabbit IgG (H+L) (Proteintech, China, Cat#SA00001‐2) for 1 h. The immunoblots were detected using an enhanced chemiluminescence (ECL) kit (Advansta, USA Cat#K‐12045‐D10) and analyzed with Image J.

    Article Title: Intermittent theta-burst stimulation promotes neurovascular unit remodeling after ischemic stroke in a mouse model
    Article Snippet: Briefly, 30 μg of denatured protein was separated by constant voltage (150 V) electrophoresis on a sodium dodecyl sulfate polyacrylamide gel electrophoresis gel (10%, Epizyme Biotech, Shanghai, China) and then transferred to a polyvinylidene fluoride membrane (Millipore, Billerica , MA, USA) by constant current (280 MA). .. After blocking with 5% bovine serum albumin (Epizyme Biotech) for 1 hour at room temperature, the membrane was incubated overnight at 4°C with the following primary antibodies: anti-PI3K (rabbit, 1:1000, Affinity, Shanghai, China, Cat# AF6241, RRID: AB_2835340), anti-AKT (rabbit, 1:1000, Affinity, Cat# AF6261, RRID: AB_2835121), anti-GSK3β (rabbit, 1:1000, Affinity, Cat# AF5016, RRID: AB_2834935), anti-phospho-AKT[Ser473] (rabbit, 1:1000, Affinity, Cat# AF0016, RRID: AB_2810275), anti-phospho-GSK3β[Ser9] (rabbit, 1:1000, Affinity, Cat# AF2016, RRID: AB_2834439), anti-β-catenin (rabbit, 1:5000, Proteintech, Wuhan, China, Cat# 51067-2-AP, RRID: AB_2086128), anti-BDNF (rabbit, 1:1000, Affinity, Cat# DF6387, RRID: AB_2838350), antizonula occludens protein 1 (ZO-1; rabbit, 1:1000, Affinity, Cat# AF5145, RRID: AB_2837631), anti-occludin (rabbit, 1:1000, Affinity, Cat# DF7504, RRID: AB_2841004), anti-claudin-5 (rabbit, 1:1000, Affinity, Cat# AF5216, RRID: AB_2837702), anti-β-actin (mouse, 1:5000, Affinity, Cat# T0022, RRID: AB_2839417), anti-β-actin (rabbit, 1:5000, Affinity, Cat# AF7018, RRID: AB_2839420), anti-Bcl-2 (rabbit, 1:2000, Proteintech, Cat# 12789-1- AP, RRID: AB_2227948), anti-Bax (mouse, 1:5000, Proteintech, Cat# 60267- 1-Ig, RRID: AB_2848213), anti-caspase 3 (rabbit, 1:1000, Proteintech, Cat# 19677-1-AP, RRID: AB_10733244), anti-vascular endothelial growth factor (VEGF; rabbit, 1:1000, Proteintech, Cat# 19003-1-AP, RRID: AB_2212657), anti-glial fibrillary acidic protein (GFAP; mouse, 1:2000, Proteintech, Cat# 60190-1-Ig, RRID: AB_10838694), anti-interleukin (IL)-1β (mouse, 1:1000, Proteintech, Cat# 66737-1-Ig, RRID: AB_2882087), anti-tumor necrosis factor alpha (TNF-α; mouse, 1:1000, Proteintech, Cat# 60291-1-Ig, RRID: AB_2833255), anti-IL-10 (mouse, 1:2000, Proteintech, Cat# 60269-1-Ig, RRID: AB_2881389), anti-nuclear factor kappa B (NF-κB; rabbit, 1:1000, Cell Signaling Technology, Danvers, MA, USA, Cat# 8242, RRID: AB_10859369), anti-phosphorylated NF-κB (p-NF-κB; rabbit, 1:1000, Cell Signaling Technology, Cat# 3033, RRID: AB_331284), anti-hypoxia-inducible factor 1α (HIF-1α; rabbit, 1:1000, Cell Signaling Technology, Cat# 36169, RRID: AB_2799095), anti-C3d (goat, 1:1000, R&D Systems, Minneapolis, MN, USA, Cat# AF2655, RRID: AB_2066622), and anti-S100A10 (rabbit, 1:1000, Proteintech, Cat# 11250-1-AP, RRID: AB_2269906). ..

    Article Title: Astroglial connexin 43 is a novel therapeutic target for chronic multiple sclerosis model
    Article Snippet: Subsequently, 10 μg protein per well was segregated using a 4.5%–15% gradient poly-acrylamide gel (Bio-Rad Laboratories) electrophoresis technique and transferred onto polyvinyl difluoride membranes. .. The membranes were first incubated with a blocking solution and then with the following primary antibodies: anti-C3d (HM1045; R&D Systems, Minneapolis, MN, USA), anti-S100A10 (AF2377; R&D Systems), anti-S100β (ab52642; Abcam), and anti-GFAP (2E1.E9; STEMCELL Technologies, Vancouver, Canada). ..

    Blocking Assay:

    Article Title: Intermittent theta-burst stimulation promotes neurovascular unit remodeling after ischemic stroke in a mouse model
    Article Snippet: Briefly, 30 μg of denatured protein was separated by constant voltage (150 V) electrophoresis on a sodium dodecyl sulfate polyacrylamide gel electrophoresis gel (10%, Epizyme Biotech, Shanghai, China) and then transferred to a polyvinylidene fluoride membrane (Millipore, Billerica , MA, USA) by constant current (280 MA). .. After blocking with 5% bovine serum albumin (Epizyme Biotech) for 1 hour at room temperature, the membrane was incubated overnight at 4°C with the following primary antibodies: anti-PI3K (rabbit, 1:1000, Affinity, Shanghai, China, Cat# AF6241, RRID: AB_2835340), anti-AKT (rabbit, 1:1000, Affinity, Cat# AF6261, RRID: AB_2835121), anti-GSK3β (rabbit, 1:1000, Affinity, Cat# AF5016, RRID: AB_2834935), anti-phospho-AKT[Ser473] (rabbit, 1:1000, Affinity, Cat# AF0016, RRID: AB_2810275), anti-phospho-GSK3β[Ser9] (rabbit, 1:1000, Affinity, Cat# AF2016, RRID: AB_2834439), anti-β-catenin (rabbit, 1:5000, Proteintech, Wuhan, China, Cat# 51067-2-AP, RRID: AB_2086128), anti-BDNF (rabbit, 1:1000, Affinity, Cat# DF6387, RRID: AB_2838350), antizonula occludens protein 1 (ZO-1; rabbit, 1:1000, Affinity, Cat# AF5145, RRID: AB_2837631), anti-occludin (rabbit, 1:1000, Affinity, Cat# DF7504, RRID: AB_2841004), anti-claudin-5 (rabbit, 1:1000, Affinity, Cat# AF5216, RRID: AB_2837702), anti-β-actin (mouse, 1:5000, Affinity, Cat# T0022, RRID: AB_2839417), anti-β-actin (rabbit, 1:5000, Affinity, Cat# AF7018, RRID: AB_2839420), anti-Bcl-2 (rabbit, 1:2000, Proteintech, Cat# 12789-1- AP, RRID: AB_2227948), anti-Bax (mouse, 1:5000, Proteintech, Cat# 60267- 1-Ig, RRID: AB_2848213), anti-caspase 3 (rabbit, 1:1000, Proteintech, Cat# 19677-1-AP, RRID: AB_10733244), anti-vascular endothelial growth factor (VEGF; rabbit, 1:1000, Proteintech, Cat# 19003-1-AP, RRID: AB_2212657), anti-glial fibrillary acidic protein (GFAP; mouse, 1:2000, Proteintech, Cat# 60190-1-Ig, RRID: AB_10838694), anti-interleukin (IL)-1β (mouse, 1:1000, Proteintech, Cat# 66737-1-Ig, RRID: AB_2882087), anti-tumor necrosis factor alpha (TNF-α; mouse, 1:1000, Proteintech, Cat# 60291-1-Ig, RRID: AB_2833255), anti-IL-10 (mouse, 1:2000, Proteintech, Cat# 60269-1-Ig, RRID: AB_2881389), anti-nuclear factor kappa B (NF-κB; rabbit, 1:1000, Cell Signaling Technology, Danvers, MA, USA, Cat# 8242, RRID: AB_10859369), anti-phosphorylated NF-κB (p-NF-κB; rabbit, 1:1000, Cell Signaling Technology, Cat# 3033, RRID: AB_331284), anti-hypoxia-inducible factor 1α (HIF-1α; rabbit, 1:1000, Cell Signaling Technology, Cat# 36169, RRID: AB_2799095), anti-C3d (goat, 1:1000, R&D Systems, Minneapolis, MN, USA, Cat# AF2655, RRID: AB_2066622), and anti-S100A10 (rabbit, 1:1000, Proteintech, Cat# 11250-1-AP, RRID: AB_2269906). ..

    Article Title: Astroglial connexin 43 is a novel therapeutic target for chronic multiple sclerosis model
    Article Snippet: Subsequently, 10 μg protein per well was segregated using a 4.5%–15% gradient poly-acrylamide gel (Bio-Rad Laboratories) electrophoresis technique and transferred onto polyvinyl difluoride membranes. .. The membranes were first incubated with a blocking solution and then with the following primary antibodies: anti-C3d (HM1045; R&D Systems, Minneapolis, MN, USA), anti-S100A10 (AF2377; R&D Systems), anti-S100β (ab52642; Abcam), and anti-GFAP (2E1.E9; STEMCELL Technologies, Vancouver, Canada). ..

    Membrane:

    Article Title: Intermittent theta-burst stimulation promotes neurovascular unit remodeling after ischemic stroke in a mouse model
    Article Snippet: Briefly, 30 μg of denatured protein was separated by constant voltage (150 V) electrophoresis on a sodium dodecyl sulfate polyacrylamide gel electrophoresis gel (10%, Epizyme Biotech, Shanghai, China) and then transferred to a polyvinylidene fluoride membrane (Millipore, Billerica , MA, USA) by constant current (280 MA). .. After blocking with 5% bovine serum albumin (Epizyme Biotech) for 1 hour at room temperature, the membrane was incubated overnight at 4°C with the following primary antibodies: anti-PI3K (rabbit, 1:1000, Affinity, Shanghai, China, Cat# AF6241, RRID: AB_2835340), anti-AKT (rabbit, 1:1000, Affinity, Cat# AF6261, RRID: AB_2835121), anti-GSK3β (rabbit, 1:1000, Affinity, Cat# AF5016, RRID: AB_2834935), anti-phospho-AKT[Ser473] (rabbit, 1:1000, Affinity, Cat# AF0016, RRID: AB_2810275), anti-phospho-GSK3β[Ser9] (rabbit, 1:1000, Affinity, Cat# AF2016, RRID: AB_2834439), anti-β-catenin (rabbit, 1:5000, Proteintech, Wuhan, China, Cat# 51067-2-AP, RRID: AB_2086128), anti-BDNF (rabbit, 1:1000, Affinity, Cat# DF6387, RRID: AB_2838350), antizonula occludens protein 1 (ZO-1; rabbit, 1:1000, Affinity, Cat# AF5145, RRID: AB_2837631), anti-occludin (rabbit, 1:1000, Affinity, Cat# DF7504, RRID: AB_2841004), anti-claudin-5 (rabbit, 1:1000, Affinity, Cat# AF5216, RRID: AB_2837702), anti-β-actin (mouse, 1:5000, Affinity, Cat# T0022, RRID: AB_2839417), anti-β-actin (rabbit, 1:5000, Affinity, Cat# AF7018, RRID: AB_2839420), anti-Bcl-2 (rabbit, 1:2000, Proteintech, Cat# 12789-1- AP, RRID: AB_2227948), anti-Bax (mouse, 1:5000, Proteintech, Cat# 60267- 1-Ig, RRID: AB_2848213), anti-caspase 3 (rabbit, 1:1000, Proteintech, Cat# 19677-1-AP, RRID: AB_10733244), anti-vascular endothelial growth factor (VEGF; rabbit, 1:1000, Proteintech, Cat# 19003-1-AP, RRID: AB_2212657), anti-glial fibrillary acidic protein (GFAP; mouse, 1:2000, Proteintech, Cat# 60190-1-Ig, RRID: AB_10838694), anti-interleukin (IL)-1β (mouse, 1:1000, Proteintech, Cat# 66737-1-Ig, RRID: AB_2882087), anti-tumor necrosis factor alpha (TNF-α; mouse, 1:1000, Proteintech, Cat# 60291-1-Ig, RRID: AB_2833255), anti-IL-10 (mouse, 1:2000, Proteintech, Cat# 60269-1-Ig, RRID: AB_2881389), anti-nuclear factor kappa B (NF-κB; rabbit, 1:1000, Cell Signaling Technology, Danvers, MA, USA, Cat# 8242, RRID: AB_10859369), anti-phosphorylated NF-κB (p-NF-κB; rabbit, 1:1000, Cell Signaling Technology, Cat# 3033, RRID: AB_331284), anti-hypoxia-inducible factor 1α (HIF-1α; rabbit, 1:1000, Cell Signaling Technology, Cat# 36169, RRID: AB_2799095), anti-C3d (goat, 1:1000, R&D Systems, Minneapolis, MN, USA, Cat# AF2655, RRID: AB_2066622), and anti-S100A10 (rabbit, 1:1000, Proteintech, Cat# 11250-1-AP, RRID: AB_2269906). ..



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    ( A ) Levels of C3d bound to B cells 2 days after the induction of DOX-induced allograft club cell injury. Representative FACS histogram of C3d B cell staining and (right panel) plots of C3d MFI normalized to a corresponding fluorescence minus one (FMO) control ( n = 4/group). ( B ) POD 16 FACS contour plot of intragraft B cell abundance (left), ki67 histogram (proliferation) (middle), and a plot of total allograft B cell numbers on POD 16 (right). FACS contour plots and histograms are representative results from 4/group. ( C ) Percentages of BAL CD19 + lymphocytes in UCSF cohorts who carry the GG genotype and C3 R102G (CC&GC) polymorphisms. P = 0.02 by an unpaired t test. ( D ) Quantitation of BAL B cell numbers in POD 16 allografts ( n = 4/group). Levels of POD 16 of ( E ) BAL ( n = 4/group) and ( F ) serum ( n = 5/group) donor reactive IgM, IgG 1 , IgG 2c , and IgG 3 . ( G ) POD 16, intragraft CD138 + IgM + and IgG + Ab-secreting cell numbers ( n = 5/group). ( H ) A representative FACS gating strategy used to identify intragraft CD80 + CD73 + CD273 + memory B cells, as shown in the bar graphs ( I ), which display the total intragraft numbers of these cells expressing either surface IgM ( n = 5/group) or IgG ( n = 4/group) on POD 16. Bar graphs and dot blots show mean ± SD values according to 2-way ANOVA with Šidák’s multiple comparisons test ( A ), and Welch’s t test ( B , D – G , and I ). * P < 0.05, ** P < 0.01, *** P < 0.001.

    Journal: The Journal of Clinical Investigation

    Article Title: Impaired complement regulation drives chronic lung allograft dysfunction after lung transplantation

    doi: 10.1172/JCI188891

    Figure Lengend Snippet: ( A ) Levels of C3d bound to B cells 2 days after the induction of DOX-induced allograft club cell injury. Representative FACS histogram of C3d B cell staining and (right panel) plots of C3d MFI normalized to a corresponding fluorescence minus one (FMO) control ( n = 4/group). ( B ) POD 16 FACS contour plot of intragraft B cell abundance (left), ki67 histogram (proliferation) (middle), and a plot of total allograft B cell numbers on POD 16 (right). FACS contour plots and histograms are representative results from 4/group. ( C ) Percentages of BAL CD19 + lymphocytes in UCSF cohorts who carry the GG genotype and C3 R102G (CC&GC) polymorphisms. P = 0.02 by an unpaired t test. ( D ) Quantitation of BAL B cell numbers in POD 16 allografts ( n = 4/group). Levels of POD 16 of ( E ) BAL ( n = 4/group) and ( F ) serum ( n = 5/group) donor reactive IgM, IgG 1 , IgG 2c , and IgG 3 . ( G ) POD 16, intragraft CD138 + IgM + and IgG + Ab-secreting cell numbers ( n = 5/group). ( H ) A representative FACS gating strategy used to identify intragraft CD80 + CD73 + CD273 + memory B cells, as shown in the bar graphs ( I ), which display the total intragraft numbers of these cells expressing either surface IgM ( n = 5/group) or IgG ( n = 4/group) on POD 16. Bar graphs and dot blots show mean ± SD values according to 2-way ANOVA with Šidák’s multiple comparisons test ( A ), and Welch’s t test ( B , D – G , and I ). * P < 0.05, ** P < 0.01, *** P < 0.001.

    Article Snippet: Cell surface staining was performed with the following Abs: CD45 (clone 30-F11; eBioscience), CD45.2 (clone 104; BioLegend), CD90.2 (clone 53-2.1; eBioscience), CD4 (clone RM4-5; eBioscience), CD8α (clone 53-6.7; eBioscience), B220 (RA3-6B2; Biolegend), CD19 (1D3; BD Biosciences), rabbit anti–human/mouse/rat C3d (catalog bs-4877R; Bioss), CD138 (281-2; Biolegend) CD38 (S21016F; Biolegend), IgD (11-26c.2a; Biolegend), Foxp3 (FJK-16s; Thermo Fisher), CD73 (TY/11.8; Biolegend), CD80 (16-10A1; Biolegend), CD273 (TY25; Thermo Fisher), CD31 (clone 390; BioLegend), CD34 (clone HM34; BioLegend), and CD326 (clone G8.8; BioLegend).

    Techniques: Staining, Fluorescence, Control, Quantitation Assay, Expressing

    C3d and CD19 immunofluorescent staining of Crry –/– ( A ) and wild-type Crry +/+ ( B ) recipient allograft tissue (LTx). Stars and arrows denote C3d staining on vascular endothelium and bronchial epithelium, respectively. Rectangular insets depict tertiary lymphoid aggregates enriched with B cells. Scale bars: 150 μm. The images shown are representative results of at least 3 transplants per group.

    Journal: The Journal of Clinical Investigation

    Article Title: Impaired complement regulation drives chronic lung allograft dysfunction after lung transplantation

    doi: 10.1172/JCI188891

    Figure Lengend Snippet: C3d and CD19 immunofluorescent staining of Crry –/– ( A ) and wild-type Crry +/+ ( B ) recipient allograft tissue (LTx). Stars and arrows denote C3d staining on vascular endothelium and bronchial epithelium, respectively. Rectangular insets depict tertiary lymphoid aggregates enriched with B cells. Scale bars: 150 μm. The images shown are representative results of at least 3 transplants per group.

    Article Snippet: Cell surface staining was performed with the following Abs: CD45 (clone 30-F11; eBioscience), CD45.2 (clone 104; BioLegend), CD90.2 (clone 53-2.1; eBioscience), CD4 (clone RM4-5; eBioscience), CD8α (clone 53-6.7; eBioscience), B220 (RA3-6B2; Biolegend), CD19 (1D3; BD Biosciences), rabbit anti–human/mouse/rat C3d (catalog bs-4877R; Bioss), CD138 (281-2; Biolegend) CD38 (S21016F; Biolegend), IgD (11-26c.2a; Biolegend), Foxp3 (FJK-16s; Thermo Fisher), CD73 (TY/11.8; Biolegend), CD80 (16-10A1; Biolegend), CD273 (TY25; Thermo Fisher), CD31 (clone 390; BioLegend), CD34 (clone HM34; BioLegend), and CD326 (clone G8.8; BioLegend).

    Techniques: Staining